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论文类型：期刊论文

发表时间：2013-05-15

发表刊物：BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

收录刊物：SCIE、PubMed、Scopus

卷号：23

期号：10

页面范围：2916-2919

ISSN号：0960-894X

关键字：Fluorescence; Probe; Cu2+; Rhodamine; Cell imaging

摘要：A novel rhodamine spirolactam derivative 3',6'-Bis(diethylamino)-2-(2-hydroxyethylamino) spiro[iso-indoline-1,9'-xanthen]-3-one (RO1) was synthesized, and characterized by high-resolution mass spectrometry (HRMS), X-ray crystallography, Infrared spectroscopy (IR), and H-1 NMR and C-13 NMR spectroscopy. RO1 exhibited highly sensitive and exclusively selective fluorescence response toward Cu2+ over other metal ions with a detection limit of 0.56 ppb in mixed aqueous solution. The fluorescence was pH-independent in the wide range pH 3.1-11.6. The turn-on fluorescence enhancement of the probe is based on Cu2+ induced ring-opening mechanism of the rhodamine spirolactam. Moreover, by means of fluorescence microscopy experiments, it was demonstrated that RO1 could monitor trace Cu2+ changes by live cell imaging. (C) 2013 Elsevier Ltd. All rights reserved.