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    谢健

    • 高级工程师      
    • 性别:男
    • 毕业院校:大连工学院夜大
    • 所在单位:生物工程学院
    • 电子邮箱:xiejian@dlut.edu.cn

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    Isolation and characterization of an Alcaligenes sp strain DG-5 capable of degrading DDTs under aerobic conditions

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    论文类型:期刊论文

    发表时间:2011-07-01

    发表刊物:JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES

    收录刊物:SCIE、PubMed、Scopus

    卷号:46

    期号:3

    页面范围:257-263

    ISSN号:0360-1234

    关键字:DDTs; biodegradation; aerobic degradation; Alcaligenes sp.

    摘要:In this study, an Alcaligenes sp. strain DG-5 that can effectively degrade dichlorodiphenyltrichloro-ethanes (DDTs) under aerobic conditions was isolated from DDTs-contaminated sediment. Various factors that affect the biodegradation of DDTs by DG-5 were investigated. About 88 %, 65 % and 45 % of the total DDTs were consumed within 120 h when their initial concentrations were 0.5, 5 and 15 mg L(-1), respectively. However, almost no degradation was observed when their concentration was increased to 30 mg L(-1), but the addition of nutrients significantly improved the degradation, and 66 % and 90 % of the total DDTs were degraded at 336 h in the presence of 5 g L(-1) peptone and yeast extract, respectively. Moreover, the addition of 20 mM formate also enhanced the ability of DG-5 to transform DDTs, and its DDT transformation capacity (T(c)) value was increased by 1.8 - 2.7 fold for the pure (p,p'-DDT or o,p'-DDT only) and mixed systems (p,p'-DDT, o,p'-DDT, p,p'-DDD and p,p'-DDE). Furthermore, it was found that competitive inhibition in the biodegradation by DDT compounds occurred in the mixed system.