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Indexed by:期刊论文

Date of Publication:1999-10-28

Journal:应用与环境生物学报

Volume:5

Issue:5

Page Number:512-514

ISSN No.:1006-687X

Key Words:RubisCO;沼泽红假单胞菌;工程菌;构建

Abstract:构建了含有RubisCO Form II基因的可转化大肠杆菌-紫色非硫杆菌穿梭质粒(PMPB-2),将其转化紫色非硫杆菌野生型Rhodopseudomonas palustris No.7和紫色非硫杆菌RubisCO Form I缺陷型Rhodopseudomonas palustris No.7-DFI,获得2株RubisCO基因工程菌MG11和MG14,二者的RubisCO酶活性比对照菌分别提高76.7%和83.3%.连续传40代表明,重组质粒在受体菌中是较稳定的.