刘天庆

个人信息Personal Information

教授

博士生导师

硕士生导师

性别:男

毕业院校:大连理工大学

学位:博士

所在单位:化工学院

学科:化学工程. 生物医学工程

办公地点:1. 西部校区D段楼305
2. 环境生命楼3-403

联系方式:Email: liutq@dlut.edu.cn QQ: 343028655

电子邮箱:liutq@dlut.edu.cn

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In Vitro Fabrication and Biocompatibility Assay of a Biomimetic Osteoblastic Niche

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论文类型:期刊论文

发表时间:2019-10-01

发表刊物:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录刊物:SCIE、PubMed

卷号:189

期号:2

页面范围:471-484

ISSN号:0273-2289

关键字:Osteoblasts; Biomimetic niche; Hematopoietic stem cell expansion; Dynamic environment; Cell function maintain

摘要:A novel HAp-CS/Gel biomimetic osteoblastic niche was fabricated by freeze-drying, and its mechanical strength and biocompatibility were characterized. HAp-CS/Gel scaffolds in various ratios of 100:5, 100:10, and 100:20 (CS/Gel to HAp) were prepared by freeze-drying prior to chemical cross-linking followed by infrared spectrum analysis, EDS, FITR, SEM, fluorescence microscopy, MTT, and ALP experiments. Results from the infrared spectrum analysis showed that HAp doping remained the surface morphology and the architecture of scaffold with interconnected pores in the size range of 135 to 150 mu m. The HAp doping ratio of 100:20 was found to be optimal based on its high porosity of 90%, better water uptake folds of 19.1. In addition, EDS and FITR analyses demonstrated that HAps were uniformly distributed on the surface of a scaffold with aggregates and particles, which has sufficient roughness for cell attachment and proliferation of osteoblasts. Under SEM and fluorescent microscopy, osteoblasts seeded onto the scaffold showed evenly distributed viable cells, which is believed to form a biomimetic niche. In the present study, we further demonstrate that osteoblasts can maintain their function and grow well on the scaffold through MTT and ALP tests. Thus, the scaffold has favorable physical properties and biocompatibility to support the proliferation and differentiation of osteoblasts and further to support the constructs of biomimetic osteoblastic niche.