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'欧美杨107'组培苗瓶外生根

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Indexed by:期刊论文

Date of Publication:2010-01-01

Journal:植物生理学通讯

Included Journals:Scopus、PKU、ISTIC、CSCD

Volume:46

Issue:10

Page Number:1050-1054

ISSN No.:0412-0922

Key Words:'欧美杨107'; 组培苗; 瓶外生根; 生根率; 移栽

Abstract:'欧美杨107'是近年来重点推广的杨树品种之一,但其组培苗存在移栽成活率不高的问题.本试验研究了'欧美杨107'组培苗的直接瓶外生根和瓶内复壮后
   瓶外生根两种生根方式,结果表明,两种方式蘸取IBA的最佳浓度分别为10mg·L-1和100mg·L-1,生根率分别达73.33%和81.67%,
   移栽成活率分别为62.5%和93.33%.进一步研究发现生根的过程中外源补加0.5mg·L-1IBA溶液能加速生根,使生根周期缩短2周左右.添加
   1/2MS营养液可促进生根苗茎的生长和根的伸长,有利于移栽成活.瓶内生根苗的生根率高达到95%,但是移栽难以成活,显微镜观察发现瓶外生根苗比瓶内
   生根苗的根毛发达,验证了瓶外生根苗的根吸收功能更好.该研究对促进'欧美杨107'组培苗及其转基因苗的推广应用具有重要的现实意义

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