贾凌云

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教授

博士生导师

硕士生导师

主要任职:生物工程学院院长、党委副书记

性别:女

毕业院校:大连理工大学

学位:博士

所在单位:生物工程学院

学科:生物工程与技术. 生物化工

办公地点:知微楼519房间

电子邮箱:lyjia@dlut.edu.cn

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Optimization of dilution refolding conditions for a camelid single domain antibody against human beta-2-microglobulin

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论文类型:期刊论文

发表时间:2016-01-01

发表刊物:PROTEIN EXPRESSION AND PURIFICATION

收录刊物:SCIE、PubMed、Scopus

卷号:117

页面范围:59-66

ISSN号:1046-5928

关键字:Beta-2-microglobulin; Single domain antibody; Dilution refolding

摘要:Single domain antibody (sdAb) is often expressed as inclusion bodies in Escherichia coli cytoplasm. Establishing an effective in vitro refolding method for sdAb obtained from inclusion bodies would be important for sdAb research. In this study, dilution refolding condition for a camelid sdAb specific against human beta-2-microglobulin was optimized for the sdAb purified from the inclusion bodies of E. coli BL21 (DE3). Single factor methods based on protein concentration, velocity of dilution, incubation time and refolding buffer composition were first investigated. Then the key refolding buffer compositions were selected for further optimization by means of the Box-Behnken design of response surface methodology (RSM). The activity of the refolded sdAb was determined by measuring its specific antigen-binding ability using indirect ELISA. The optimized refolding condition of sdAb consisted of a 10-fold dilution in 10 mM Tris-HCl (pH 8.0) containing 1.24 mM GSH, 1 mM GSSG, 352 mM L-Arg, 0.65% PEG-2000, and a 16 h incubation at 4 degrees C. Further comparison of the activities between the refolded sdAb and purified soluble sdAb expressed in E. coli Rosetta-gami (DE3) pLysS indicated that the sdAb was correctly refolded, as assayed by isothermal titration calorimetry. This work could provide an important strategy for the recombinant production and application of sdAb. (C) 2015 Elsevier Inc. All rights reserved.