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论文类型：期刊论文

发表时间：2016-04-01

发表刊物：JOURNAL OF FOOD AND DRUG ANALYSIS

收录刊物：SCIE、PubMed、Scopus

卷号：24

期号：2

页面范围：399-405

ISSN号：1021-9498

关键字：Centrifugal partition chromatography; Content determination; Urea cream

摘要：The objective of this study is to establish a centrifugal partition chromatography (CPC) method for determination of the urea ingredient in urea cream. The mechanism of this method is that urea is determined by UV detector at 430 nm after being extracted from the cream and derivatized on line via Ehrlich reaction in rotor of CPC, where the reaction products dissolve in the mobile phase and the cream matrix retains in the stationary phase. The mixed solvent consisting of n-hexane, methanol, hydrochloric acid and p-dimethylaminobenzaldehyde with a ratio of 1000 mL:1000 mL:18 mL:2.0 g is used for solvent system of CPC. The CPC method proposed offers good precision and convenience without complex sample pretreatment processes. Copyright (C) 2015, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.