袁文杰

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教授

博士生导师

硕士生导师

主要任职:生物工程学院副院长

其他任职:辽宁省生物工程教指委秘书长

性别:女

毕业院校:大连理工大学

学位:博士

所在单位:生物工程学院

学科:生物化工

办公地点:生物楼321

联系方式:0411-84706802

电子邮箱:ywj@dlut.edu.cn

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General Control Nonderepressible 2 (GCN2) Kinase Inhibits Target of Rapamycin Complex 1 in Response to Amino Acid Starvation in Saccharomyces cerevisiae

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论文类型:期刊论文

第一作者:Yuan, Wenjie

通讯作者:Jiang, Y (reprint author), Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15213 USA.

合写作者:Guo, Shuguang,Gao, Jiaoqi,Zhong, Mingming,Yan, Gonghong,Wu, Wangmeng,Chao, Yapeng,Jiang, Yu

发表时间:2017-02-17

发表刊物:JOURNAL OF BIOLOGICAL CHEMISTRY

收录刊物:SCIE、EI、PubMed

卷号:292

期号:7

页面范围:2660-2669

ISSN号:0021-9258

关键字:Gcn2,Kog1,TOR complex (TORC),TORC1,amino acid,amino acid starvation,autophagy,histidine,uncharged tRNA,yeast

摘要:In eukaryotic cells, two conserved protein kinases, Gcn2 and TOR complex 1 (TORC1), couple amino acid conditions to protein translation. Gcn2 functions as an amino acid sensor and is activated by uncharged tRNAs that accumulate when intracellular amino acids are limited. Activated Gcn2 phosphorylates and inhibits eukaryotic initiation factor-2 alpha (eIF2 alpha), resulting in repression of general protein synthesis. Like Gcn2, TORC1 is also involved in sensing amino acid conditions. However, the underlying mechanism remains unclear. In the present study, we show that TORC1 is a direct target of Gcn2 kinase in the yeast Saccharomyces cerevisiae. In response to amino acid starvation, Gcn2 binds to TORC1 and phosphorylates Kog1, the unique regulatory subunit of TORC1, resulting in down-regulation of TORC1 kinase activity. In the absence of Gcn2, TORC1 signaling activity increases and becomes unresponsive to amino acid starvation. Our findings demonstrate that TORC1 is an effector of Gcn2 in amino acid signaling, hence defining a novel mechanism by which TORC1 senses amino acid starvation.