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论文类型：期刊论文

第一作者：Gao, Jiaoqi

通讯作者：Yuan, WJ (reprint author), Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China.

合写作者：Yuan, Wenjie,Li, Yimin,Xiang, Ruijuan,Hou, Shengbo,Zhong, Shijun,Bai, Fengwu

发表时间：2015-08-14

发表刊物：BIOTECHNOLOGY FOR BIOFUELS

收录刊物：SCIE、EI、PubMed、Scopus

卷号：8

期号：1

页面范围：115

ISSN号：1754-6834

关键字：Kluyveromyces marxianus; RNA-seq; Ethanol fermentation; Inulin; Transcriptome analysis

摘要：Background: Ethanol production from non-crop materials, such as Jerusalem artichokes, would make a great contribution to the energy industry. The non-conventional yeast, Kluyveromyces marxianus, is able to carry out ethanol fermentation of sugar molecules obtained from inulin-containing materials by consolidated bioprocessing. Lower inulin concentrations and micro-aeration can lead to a relatively fast and ideal fermentation process; however, it is unclear what causes the inhibition of higher concentrations of inulin and the promotion effect of aeration.
   Results: Next-generation sequencing technology was used to study the global transcriptional response of K. marxianus Y179 under three fermentation conditions, including 120 g/L inulin without aeration (120-N), 230 g/L inulin without aeration (230-N), 230 g/L inulin with aeration by ORP controlling at -130 mV (230-130mV). A total of 35.55 million clean reads were generated from three samples, of which 4,820 predicted that open reading frames were annotated. For differential expression analysis, 950 and 1,452 differentially expressed genes were discovered under the conditions of 230-130mV and 120-N, respectively, and the sample 230-N was used as the control. These genes are mainly associated with the pathways of central carbon metabolism and ethanol formation. Increased expression of inulinase and the low activity of the autophagy-related gene, ATG8, ensured fast and ideal fermentation processes.
   Conclusions: Despite being reported as the "crabtree-negative" species, K. marxianus Y179 could achieve favorable ethanol fermentation profiles under micro-aeration and high inulin concentrations. K. marxianus Y179 cells responded to inulin concentrations and micro-aeration that is involved in the whole ethanol metabolism network. These results will serve as an important foundation for further exploration of the regulatory mechanisms involved in ethanol fermentation from inulin by consolidated bioprocessing and also provide a valuable reference for future studies on optimization and reconstruction of the metabolism network in K. marxianus.