点击次数：

论文类型：期刊论文

发表时间：2017-01-01

发表刊物：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY

收录刊物：SCIE

卷号：19

期号：5

页面范围：1265-1269

ISSN号：1560-8530

关键字：Virus infection; Cell ultrastructure; Chloroplasts; Coat protein

摘要：The symptoms of Lily mottled virus (LMoV) disease are thought to be caused by metabolic changes in leaf chloroplasts. To observe variations in the ultrastructure of cells and the accumulation of viral coat protein (CP) in lily leaves, we examined ultrathin sections of lily leaves infected by LMoV. Immunogold labeling analysis demonstrated that LMoV-CP was localized to the chloroplasts. The chlorophyll fluorescence parameters of LMoV-infected lily, which indicating that the accumulation of LMoV-CP in chloroplasts inhibits PSII activity. We investigated the transmembrane transport of LMoV CP by incubating a gradient of this protein. The lowest concentration of LMoV-CP detected was 30 mu g mL(-1) and the optimal incubation time was 1 min. High levels of LMoV-CP that accumulate inside chloroplasts may affect photosynthesis in virus-infected lily by inhibiting photosystem activity. (C) 2017 Friends Science Publishers