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论文类型：期刊论文

发表时间：2017-10-01

发表刊物：BIOTECHNOLOGY JOURNAL

收录刊物：Scopus、SCIE、EI、PubMed

卷号：12

期号：10,SI

ISSN号：1860-6768

关键字：ABE fermentation; Clostridium acetobutylicum; fructose utilization; intracellular metabolite analysis; redox potential control

摘要：Jerusalem artichoke (JA) can grow well in marginal lands with high biomass yield, and thus is a potential energy crop for biorefinery. The major biomass of JA is from tubers, which contain inulin that can be easily hydrolyzed into a mixture of fructose and glucose, but fructose utilization for producing butanol as an advanced biofuel is poor compared to glucose-based ABE fermentation by Clostridium acetobutylicum. In this article, the impact of extracellular redox potential (ORP) on the process is studied using a mixture of fructose and glucose to simulate the hydrolysate of JA tubers. When the extracellular ORP is controlled above -460 mV, 13.2 gL(-1) butanol is produced from 51.0 gL(-1) total sugars (40.1 gL(-1) fructose and 10.9 gL(-1) glucose), leading to dramatically increased butanol yield and butanol/ABE ratio of 0.26 g g(-1) and 0.67, respectively. Intracellular metabolite and q-PCR analysis further indicate that intracellular ATP and NADH availabilities are significantly improved together with the fructose-specific PTS expression at the lag phase, which consequently facilitate fructose transport, metabolic shift toward solventogenesis and carbon flux redistribution for butanol biosynthesis. Therefore, the extracellular ORP control can be an effective strategy to improve butanol production from fructose-based feedstock.