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发表时间：2004-01-01

发表刊物：大连理工大学学报

所属单位：机械工程学院

期号：4

页面范围：523-527

ISSN号：1000-8608

摘要：The development and the application of measurement for microflow characteristics are generally introduced. Exciting light sources, fluorescence microscopy and high-precision CCD camera are selected to set up the MicroPIV device, which is used to measure the electroosmosis flow characteristics in microfluidic chips, and the technique parameters and the valid ranges on the micro-imaging unit, light sources and tracking particles are provided too. According to the feature size and the electroosmosis velocity of microfluidic chips, key problems encountered in the application of MicroPIV, such as optical resolution, measurement precision, and the range of velocity measurement are analyzed in detail. When the intensity of the electric field is equal to 100 V/cm, the electroosmosis velocity of borax solution is about 220 ��m/s in a microchannel of 50 ��m width and under the magnification less than 40 times, the dynamic velocity range can be suitable for measurement of the electroosmosis flow. For a fluorescence particle of 300 nm in diameter, the imaging size is larger than that of three pixels in CCD, therefore effective measurements can be obtained. The measurement precision of MicroPIV depends on the parameters of microscope, electric force, image processing technique and Brownian motion.

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