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Indexed by:Journal Papers
Date of Publication:2021-04-01
Journal:BIOORGANIC & MEDICINAL CHEMISTRY
Volume:28
Issue:3
Page Number:115280
ISSN No.:0968-0896
Key Words:Nitroreductase (NTR); 1,8-Naphthalimide; Escherichia coli (E. coli); Staphylococcus aureus. (S. aureus)
Abstract:A nitroreductase (NTR) responsive fluorescent probe, Na-NO2, comprising p-nitrobenzyl as the unique recognition group and 1,8-naphthalimide as fluorophore, was synthesized. Na-NO2 showed remarkable fluorescence "turn-on" signal in the presence of NTR under DMSO/H2O (1:19, v/v) buffered with PBS (pH = 7) solution in the presence of NADH (300 mu M). Furthermore, the probe has a low detection limit down to 3.4 ng/mL and it is very sensitive towards the NTR in Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), normal and tumor cells such as HL-7702, HepG-2 and MCF-7.
