彭伟

个人信息Personal Information

教授

博士生导师

硕士生导师

性别:女

毕业院校:大连理工大学

学位:博士

所在单位:物理学院

学科:光学工程. 光学

办公地点:物理与光电工程学院235

联系方式:wpeng@dlut.edu.cn

电子邮箱:wpeng@dlut.edu.cn

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Boronic acid functionalized fiber-optic SPR sensors for high sensitivity glycoprotein detection

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论文类型:期刊论文

发表时间:2018-05-01

发表刊物:SENSORS AND ACTUATORS B-CHEMICAL

收录刊物:SCIE、EI

卷号:260

页面范围:976-982

ISSN号:0925-4005

关键字:Phenylboronic acid; Fiber-optic SPR sensors; Glycoprotein detection; Regeneration

摘要:Boronic acid and its derivatives have shown special capability to cis-diols groups such as glycoprotein and carbohydrate, which has been widely investigated and applied in practical disease diagnosis. Fiberoptic surface plasmon resonance (SPR) sensors with tiny size are very accurate and convenient tools for monitoring the surface interaction in real time. In this paper, phenylboronic acids (PBA) were formed on the sensing surface by coating the fiber surface with a self-assembled monolayer (SAM) with long and short alkyl groups. Compared with commercial available 4-mercaptophenyboronic acid, PBA with long carbon chain provided more flexible space in binding process and achieved much higher sensitivity to glycoprotein. Furthermore, PBA SAM showed much stronger response for Concanavalin A (ConA) than the traditional protein-protein interaction. The limit of detection for ConA was as low as 0.29 nM by L-PBA SAM modified fiber-optic SPR sensors. Finally, we tested different regeneration solutions to remove glycoprotein from the sensor surface and found that only acidic environment was not enough and the protein denaturant such as ethanol or urea was essential for glycoprotein dissociation. (C) 2018 Elsevier B.V. All rights reserved.