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论文类型：期刊论文

发表时间：2007-12-01

发表刊物：SEPARATION SCIENCE AND TECHNOLOGY

收录刊物：SCIE、EI、Scopus

卷号：42

期号：16

页面范围：3741-3757

ISSN号：0149-6395

关键字：BSA; revered micelles; extraction ratio; selectivity

摘要：The extraction and back extraction of Bovine Serum Albumin (BSA) using a new reversed micelle system of CTAB/isooctane/1-pentanol have been investigated. Under the optimal operating conditions in this study, the extraction ratio of BSA reaches as high as 98%. The back extraction ratio can reach 80% as pH in stripping phase approaches to the isoelectric point (pI) of BSA. UV-spectra indicated that there was no significant change in the structure of BSA enriched in stripping phase. Furthermore, the reversed micelle system was repeatedly used to extract BSA from a mixed solution of protein.