大连理工大学  登录  English 
于海宁
点赞:

副教授   博士生导师   硕士生导师

主要任职: 无

性别: 女

毕业院校: 美国爱荷华大学

学位: 博士

在职信息:在职

所在单位: 生物工程学院

学科: 生物化学与分子生物学 细胞生物学

办公地点: 大连理工大学老校区,老化学楼(生命楼南)117

联系方式: joannyu@live.cn;yuhaining@dlut.edu.cn

电子邮箱:

手机版

访问量:

开通时间 : ..

最后更新时间: ..

当前位置: 于海宁 >> 科学研究 >> 论文成果
Effects of C-terminal amidation and heptapeptide ring on the biological activities and advanced structure of amurin-9KY, a novel antimicrobial peptide identified from the brown frog, Rana kunyuensis

点击量:

论文类型: 期刊论文

第一作者: Zhang Fen

合写作者: Guo Zhi-Lai,Chen Yan,Li Li,Yu Hai-Ning,Wang Yi-Peng

发表时间: 2019-05-18

发表刊物: Zoological research

收录刊物: PubMed

卷号: 40

期号: 3

页面范围: 198-204

ISSN号: 2095-8137

关键字: Amurin-9KY,Antimicrobial peptides,C-terminal amidation,Heptapeptide ring,Rana kunyuensis,Structure activity relationship

摘要: Rana kunyuensis is a species of brown frog that lives exclusively on Kunyu Mountain, Yantai, China. In the current study, a 279-bp cDNA sequence encoding a novel antimicrobial peptide (AMP), designated as amurin-9KY, was cloned from synthesized double-strand skin cDNA of R. kunyuensis. The amurin-9KY precursor was composed of 62 amino acid (aa) residues, whereas the mature peptide was composed of 14 aa and contained two cysteines forming a C-terminal heptapeptide ring (Rana box domain) and an amidated C-terminus. These structural characters represent a novel amphibian AMP family. Although amurin-9KY exhibited high similarity to the already identified amurin-9AM from R. amurensis, little is known about the structures and activities of amurin-9 family AMPs so far. Therefore, amurin-9KY and its three derivatives (amurin-9KY1-3) were designed and synthesized. The structures and activities were examined to evaluate the influence of C-terminal amidation and the heptapeptide ring on the activities and structure of amurin-9KY. Results indicated that C-terminal amidation was essential for antimicrobial activity, whereas both C-terminal amidation and the heptapeptide ring played roles in the low hemolytic activity. Circular dichroism (CD) spectra showed that the four peptides adopted an a-helical conformation in THF/H2O (v/v 1:1) solution, but a random coil in aqueous solution. Elimination of the C-terminal heptapeptide ring generated two free cysteine residues with unpaired thiol groups, which greatly increased the concentration-dependent anti-oxidant activity. Scanning electron microscopy (SEM) was also performed to determine the possible bactericidal mechanisms.

辽ICP备05001357号 地址:中国·辽宁省大连市甘井子区凌工路2号 邮编:116024
版权所有:大连理工大学