大连理工大学  登录  English 
于海宁
点赞:

副教授   博士生导师   硕士生导师

主要任职: 无

性别: 女

毕业院校: 美国爱荷华大学

学位: 博士

在职信息:在职

所在单位: 生物工程学院

学科: 生物化学与分子生物学 细胞生物学

办公地点: 大连理工大学老校区,老化学楼(生命楼南)117

联系方式: joannyu@live.cn;yuhaining@dlut.edu.cn

电子邮箱:

手机版

访问量:

开通时间 : ..

最后更新时间: ..

当前位置: 于海宁 >> 科学研究 >> 论文成果
Identification and characterization of two novel cathelicidins from the frog Odorrana livida

点击量:

论文类型: 期刊论文

第一作者: Qi Ruo-Han

合写作者: Chen Yan,Guo Zhi-Lai,Zhang Fen,Fang Zheng,Huang Kai,Yu Hai-Ning,Wang Yi-Peng

发表时间: 2019-03-18

发表刊物: Zoological research

收录刊物: Scopus、PubMed

卷号: 40

期号: 2

页面范围: 94-101

ISSN号: 2095-8137

关键字: Anti-inflammatory activity,Antimicrobial activity,Antimicrobial peptides (AMPs),Cathelicidins,OL-CATHs,Odorrana livida

摘要: Antimicrobial peptides (AMPs) are a group of gene-encoded small peptides that play pivotal roles in the host immune system of multicellular organisms. Cathelicidins are an important family of AMPs that exclusively exist in vertebrates. Many cathelicidins have been identified from mammals, birds, reptiles and fish. To date, however, cathelicidins from amphibians are poorly understood. In the present study, two novel cathelicidins (OL-CATH1 and 2) were identified and studied from the odorous frog Odorrana livida. Firstly, the cDNAs encoding the OL-CATHs (780 and 735 bp in length, respectively) were successfully cloned from a lung cDNA library constructed for the frog. Multi-sequence alignment was carried out to analyze differences between the precursors of the OL-CATHs and other representative cathelicidins. Mature peptide sequences of OL-CATH1 and 2 were predicted (33 amino acid residues) and their secondary structures were determined (OL-CATH1 showed a random-coil conformation and OL-CATH2 demonstrated a-helical conformation). Furthermore, OL-CATH1 and 2 were chemically synthesized and their in vitro functions were determined. Antimicrobial and bacterial killing kinetic analyses indicated that OL-CATH2 demonstrated relatively moderate and rapid antimicrobial potency and exhibited strong anti-inflammatory activity. At very low concentrations (10 μg/mL), OL-CATH2 significantly inhibited the lipopolysaccharide (LPS)-induced transcription and production of pro-inflammatory cytokines TNF-a, IL-1b and IL-6 in mouse peritoneal macrophages. In contrast, OL-CATH1 did not exhibit any detectable antimicrobial or anti-inflammatory activities. Overall, identification of these OL-CATHs from O. livida enriches our understanding of the functions of cathelicidins in the amphibian immune system. The potent antimicrobial and anti-inflammatory activities of OL-CATH2 highlight its potential as a novel candidate in anti-infective drug development.

辽ICP备05001357号 地址:中国·辽宁省大连市甘井子区凌工路2号 邮编:116024
版权所有:大连理工大学