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论文类型：期刊论文

发表时间：2013-06-01

发表刊物：ELECTROPHORESIS

收录刊物：SCIE、PubMed、Scopus

卷号：34

期号：11,SI

页面范围：1526-1529

ISSN号：0173-0835

关键字：Differential interference contrast microscopy; Living cell; Polystyrene microparticles; Refractive index

摘要：Unlike the nanoparticles with surface plasmon resonance, the optical response of polystyrene microparticles (PSMPs) is insensitive to the chemical components of the surrounding medium under the wavelength-dependent differential interference contrast microscopy. This fact is exploited for the measurement of the refractive index of cytoplasm in this study. PSMPs of 400 nm in diameter were loaded into the cell to contact cytoplasm seamlessly, and the refractive index information of cytoplasm could be extracted by differential interference contrast microscopy operated at 420 nm illumination wavelength through the contrast analysis of PSMPs images.