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A lysosome-targeting nanosensor for simultaneous fluorometric imaging of intracellular pH values and temperature

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Indexed by:Journal Papers

First Author:Zhang, Wei

Correspondence Author:Wang, XD (reprint author), Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China.

Co-authors:Abou El-Reash, Y. G.,Ding, Longjiang,Lin, Zhenzhen,Lian, Ying,Song, Bo,Yuan, Jingli,Wang, Xu-dong

Date of Publication:2018-12-01

Journal:MICROCHIMICA ACTA

Included Journals:PubMed、SCIE、Scopus

Volume:185

Issue:12

Page Number:533

ISSN No.:0026-3672

Key Words:Silica nanoparticle; Fluorescein; Rhodamine B; Europium complex; Temperature response; pH response; Intracellular sensing; Ratiometric measurement; Core-shell

Abstract:Lysosomal pH and temperature are two crucial physiological parameters that are involved in regulating intracellular homeostasis, and their precise measurements are extremely important in understanding this process and diseases diagnosis. A lysosome-targeting nanosensor has been designed for simultaneous imaging of pH values and temperature in HeLa cells. Three dyes were covalently immobilized either inside or on silica nanoparticles. The nanosensors have an average diameter of 95nm. The large surface area of these nanomaterials provides abundant sites for multi-functionality. The surface of nanosensors has been modified with positively-charged amino groups in order to facilitate endocytosis and targeting lysosome. Fluorescein is used as the indicator probe for pH measurement, rhodamine B is the probe for temperature, and a europium complex acts as the reference dye. The dual nanosensor responds to pH values in the range from 3.0 to 9.0, and to temperature in the range from 20 to 60 degrees C. Owing to its good biocompatibility and good sensitivity, the dual nanosensor has been used to monitor changes in local pH values and temperature in the lysosome of HeLa cells.

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