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Iridium(III) Complex-Based Activatable Probe for Phosphorescent/Time-Gated Luminescent Sensing and Imaging of Cysteine in Mitochondria of Live Cells and Animals


Indexed by:Journal Papers

First Author:Du, Zhongbo

Correspondence Author:Zhang, WZ; Yuan, JL (reprint author), Dalian Univ Technol, Sch Chem, State Key Lab Fine Chem, Dalian 116024, Peoples R China.; Zhang, R (reprint author), Univ Queensland, Australian Inst Bioengn & Nanotechnol, St Lucia, Qld 4072, Australia.

Co-authors:Zhang, Run,Song, Bo,Zhang, Wenzhu,Wang, Yong-Lei,Liu, Jianping,Liu, Chaolong,Xu, Zhi Ping,Yuan, Jingli

Date of Publication:2019-01-28


Included Journals:SCIE、PubMed、Scopus



Page Number:1498-1506

ISSN No.:0947-6539

Key Words:cysteine; iridium; phosphorescence; sensing and imaging; time-gated luminescence

Abstract:This study reports an activatable iridium(III) complex probe for phosphorescence/time-gated luminescence detection of cysteine (Cys) in vitro and in vivo. The probe, [Ir(ppy)(2)(NTY-bpy)](PF6) [ppy: 2-phenylpyridine; NTY-bpy: 4-methyl-4 '-(2-nitrovinyl)-2,2 '-bipyridine], is developed by incorporating a strong electron-withdrawing group, nitroolefin, into a bipyridine ligand of the Ir-III complex. The luminescence of the probe is quenched owing to the intramolecular charge transfer (ICT) process, but switched on by a specific recognition reaction between the probe and Cys. [Ir(ppy)(2)(NTY-bpy)](PF6) shows high sensitivity and selectivity for Cys detection and good biocompatibility. The long-lived emission of [Ir(ppy)(2)(NTY-bpy)](PF6) allows time-gated luminescence analysis of Cys in cells and human sera. These properties make it convenient for the phosphorescence and time-gated luminescence imaging and flow cytometry analysis of Cys in live samples. The Cys images in cancer cells and inflamed macrophage cells reveal that [Ir(ppy)(2)(NTY-bpy)](PF6) is distributed in mitochondria after cellular internalization. Visualizations and flow cytometry analysis of mitochondrial Cys levels and Cys-mediated redox activities of live cells are achieved. By using [Ir(ppy)(2)(NTY-bpy)](PF6) as a probe, in vivo sensing and imaging of Cys in D. magna, zebrafish, and mice are then demonstrated.

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