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    王丽丽

    • 副教授     硕士生导师
    • 主要任职:无
    • 性别:女
    • 毕业院校:大阪市立大学
    • 学位:博士
    • 在职信息:在职
    • 所在单位:生物工程学院
    • 学科:生物工程与技术 微生物学
    • 办公地点:生物工程学院405
    • 电子邮箱:

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    Orthogonal test design for optimization of the extraction of essential oil from Asarum heterotropoides var. Mandshuricum and evaluation of its antibacterial activity against periodontal pathogens

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    论文类型:期刊论文

    合写作者:Wang, Lili

    发表时间:2018-11-08

    发表刊物:3 BIOTECH

    收录刊物:PubMed、SCIE、PubMed、SCIE

    卷号:8

    期号:11

    页面范围:473

    ISSN号:2190-572X

    关键字:Asarum heterotropoides var; Mandshuricum; Orthogonal experimental design; Essential oil; Antibacterial activity; Periodontal pathogens

    摘要:The optimum extraction conditions of essential oil from Asarum heterotropoides var. Mandshuricum applied by an orthogonal L-9(3(3)) test were a water-to-raw material ratio of 17, a particle size of D(95)3.8mm and an extraction time of 2h. In ingredient analysis, gas chromatography/mass spectrometry analysis identified the main components of essential oil as methyl eugenol (45.95%), safrole (17.48%) and 3,5-dimethoxytoluene (10.30%) orderly. In vitro, the minimum inhibitory concentration and minimum bactericidal concentration of essential oil were 0.01 and 0.02% against F. nucleatum, 0.04 and 0.08% against P. intermedia, and 0.005 and 0.005% against P. gingivalis. In vivo, administration of essential oil significantly suppressed alveolar bone resorption induced by F. nucleatum, with bone levels remaining comparable to non-infected mice. These results of antibacterial activity of essential oil in vitro and in vivo show the inhibition of periodontal pathogens and therapy of alveolar bone resorption in mice, suggesting that its essential oil could be a potential natural therapeutic agent for treatment of periodontitis in human beings.