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    王丽丽

    • 副教授     硕士生导师
    • 主要任职:无
    • 性别:女
    • 毕业院校:大阪市立大学
    • 学位:博士
    • 在职信息:在职
    • 所在单位:生物工程学院
    • 学科:生物工程与技术 微生物学
    • 办公地点:生物工程学院405
    • 电子邮箱:

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    Isolation and characterization of a bacteriophage and its potential to disrupt multi-drug resistant Pseudomonas aeruginosa biofilms

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    论文类型:期刊论文

    第一作者:Yuan Yuyu

    合写作者:Qu Kunli,Tan Demeng,Li Xiaoyu,Wang Lili,Cong Cong,Xiu Zhilong,Xu Yongping

    发表时间:2019-01-23

    发表刊物:Microbial pathogenesis

    收录刊物:Scopus、PubMed

    卷号:128

    页面范围:329-336

    ISSN号:1096-1208

    关键字:Bacteriophage,Biofilm,Pseudomonas aeruginosa

    摘要:A lytic Pseudomonas aeruginosa bacteriophage, vB_PaeM_LS1, was isolated and characterized herein. To examine the eligibility of bacteriophage vB_PaeM_LS1 as a therapeutic bacteriophage, we analysed its genome and compared it to similar bacteriophages. Genome of bacteriophage vB_PaeM_LS1 consisted of a linear, double-stranded DNA molecule 66,095 bp in length and with 55.7% G + C content. Neighbor-joining analysis of the large subunit terminase showed that bacteriophage vB_PaeM_LS1 had similarity to the Pbunavirus genus. The potential of the lytic bacteriophage to disrupt Pseudomonas aeruginosa biofilms was assessed by scanning electron microscopy and bacterial counts. This study revealed that the bacteriophage vB_PaeM_LS1 with its lytic effect showed a high potential impact on the inhibition of the growth of Pseudomonas aeruginosa biofilm formation.Copyright © 2019 Elsevier Ltd. All rights reserved.