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    王丽丽

    • 副教授       硕士生导师
    • 性别:女
    • 毕业院校:大阪市立大学
    • 学位:博士
    • 所在单位:生物工程学院
    • 学科:生物工程与技术. 微生物学
    • 办公地点:生物工程学院405
    • 电子邮箱:wanglili@dlut.edu.cn

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    Determination of Melamine in Food by SPE and CZE with UV Detection

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    论文类型:期刊论文

    第一作者:Meng, Liang

    通讯作者:Shen, GJ (reprint author), Dalian Univ Technol, Coll Environm & Chem Engn, Dalian 116622, Peoples R China.

    合写作者:Shen, Guijun,Hou, Xiaolan,Wang, Lili

    发表时间:2009-09-01

    发表刊物:CHROMATOGRAPHIA

    收录刊物:SCIE

    卷号:70

    期号:5-6

    页面范围:991-994

    ISSN号:0009-5893

    关键字:Capillary zone electrophoresis; Solid-phase extraction; Food analysis; Melamine

    摘要:A novel method for the determination of melamine residue in food was developed using solid-phase extraction and capillary zone electrophoresis with UV detection. Spiked samples were extracted with 1% trichloroacetic acid while 0.03 g sodium deoxycholate was used to precipitate protein in the real samples. After centrifuging and clean-up by solid-phase extraction cartridge, the extract was directly analyzed by CZE-UV. The method was validated and good results were obtained with respect to precision, repeatability and spiked recovery. The limit of detection for melamine varied between 0.25 and 0.5 mg kg(-1). The proposed method was successfully applied for the analysis of melamine in food with total recoveries ranging from 94 to 102% in the spiked range of 0.5-5 mg kg(-1), and the relative standard deviations were between 1.5 and 4.1%.