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论文类型：期刊论文

发表时间：2021-01-10

发表刊物：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

卷号：59

期号：45

页面范围：20215-20223

ISSN号：1433-7851

关键字：Gibbs free energy; rhodamines; single-molecule localization microscopy; spontaneously blinking dyes; super-resolution imaging

摘要：Herein, we reported a simple, fast, and quantitative theoretical descriptor Delta G(C-O)that allows accurate predictions of a wide range of spontaneously blinking rhodamines. Delta G(C-O)denotes the Gibbs free energy differences between the closed and open forms of rhodamines and has a good linear relationship with experimental pK(cycl)values. This correlation affords an effective guide for the quantitative designs of spontaneously blinking rhodamines and eliminates trial-and-error. We have validated the predictive power of Delta G(C-O)via the development of two spontaneously blinking rhodamines of different colors and enhanced brightness. We also demonstrated their super-resolution imaging utilities in dynamic live-cell imaging. We expect that Delta G(C-O)will greatly facilitate the efficient creations of spontaneously blinking fluorophores and aid the advancements of super-resolution bioimaging techniques.