• 更多栏目

    迟占有

    • 教授     博士生导师   硕士生导师
    • 性别:男
    • 毕业院校:华东理工大学
    • 学位:博士
    • 所在单位:生物工程学院
    • 学科:生物化工. 化学工程
    • 办公地点:大连理工大学 生物楼356
    • 联系方式:13234053986
    • 电子邮箱:chizhy@dlut.edu.cn

    访问量:

    开通时间:..

    最后更新时间:..

    An Extended Approach to Quantify Triacylglycerol in Microalgae by Characteristic Fatty Acids

    点击次数:

    论文类型:期刊论文

    第一作者:Yang, Miao

    通讯作者:Xue, S (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian, Peoples R China.

    合写作者:Fan, Yan,Wu, Pei-Chun,Chu, Ya-Dong,Shen, Pei-Li,Xue, Song,Chi, Zhan-You

    发表时间:2017-11-13

    发表刊物:FRONTIERS IN PLANT SCIENCE

    收录刊物:SCIE、PubMed

    卷号:8

    页面范围:1949

    ISSN号:1664-462X

    关键字:microalgae; triacylglycerol; quantification; characteristic fatty acid; linear correlation

    摘要:Microalgae represent a third generation biofuel feedstock due to their high triacylglycerol (TAG) content under adverse environmental conditions. Microalgal TAG resides in a single cell and serves as a lipid class mixed with complicated compositions. We previously showed that TAG possessed characteristic fatty acids (CFAs) for quantification and was linearly correlated with the relative abundance of CFA within certain limits in microalgae. Here, we defined the application range of the linear correlation between TAG and CFA in the oleaginous microalgae Chlamydomonas reinhardtii and Phaeodactylum tricornutum. In addition, TAG quantification was further expanded to a wide range of levels and the absolute amounts of saturated or monounsaturated CFAs, 16:0 and 18:1n9 of C. reinhardtii and 16:0 and 16:1n7 of P. tricornutum, instead of polyunsaturated CFAs, were verified to be linearly correlated to TAG levels throughout the entire period of nitrogen stress. This approach utilizes a single fatty acid to quantify TAG mixtures, and is rapid, simple and precise, which provides a useful tool for monitoring TAG accumulation of distinct microalgal species and facilitating high-throughput mutant screening for microalgae.