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论文类型： 期刊论文

第一作者： Liu, Meng

通讯作者： Brennan, JD; Li, YF (reprint author), McMaster Univ, Dept Biochem & Biomed Sci, 1280 Main St West, Hamilton, ON L8S 4K1, Canada.; Brennan, JD; Li, YF (reprint author), McMaster Univ, Biointerfaces Inst, Hamilton, ON L8S 4O3, Canada.

合写作者： Zhang, Qiang,Brennan, John D.,Li, Yingfu

发表时间： 2018-09-01

发表刊物： MRS COMMUNICATIONS

收录刊物： SCIE

卷号： 8

期号： 3

页面范围： 687-694

ISSN号： 2159-6859

摘要： Herein we describe the use of a new DNAzyme/graphene hybrid material as a biointerfaced sensing platform for optical detection of pathogenic bacteria. The hybrid consists of a colloidal graphene nanomaterial and an Escherichia coli-activated RNA-cleaving DNAzyme and is prepared via non-covalent self-assembly of the DNAzyme onto the graphene surface. Exposure of the hybrid material to E. coli-containing samples results in the release of the DNAzyme, followed by the cleavage-mediated production of a fluorescent signal. Given that specific RNA-cleaving DNAzymes can be created for diverse bacterial pathogens. direct interfacing of graphene materials with such DNAzymes represents a general and attractive approach for real-time, sensitive, and highly selective detection of pathogenic bacteria.