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论文类型： 期刊论文

发表时间： 2019-06-11

发表刊物： ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录刊物： SCIE、PubMed、EI

卷号： 58

期号： 24

页面范围： 8013-8017

ISSN号： 1433-7851

关键字： aptamers; biosensors; DNA; glutamate dehydrogenase; rolling circle amplification

摘要： We report on the first effort to select DNA aptamers from a circular DNA library, which resulted in the discovery of two high-affinity circular DNA aptamers that recognize the glutamate dehydrogenase (GDH) from Clostridium difficile, an established antigen for diagnosing Clostridium difficile infection (CDI). One aptamer binds effectively in both the circular and linear forms, the other is functional only in the circular configuration. Interestingly, these two aptamers recognize different epitopes on GDH, demonstrating the advantage of selecting aptamers from circular DNA libraries. A sensitive diagnostic test was developed to take advantage of the high stability of circular DNA aptamers in biological samples and their compatibility with rolling circle amplification. This test is capable of identifying patients with active CDI using stool samples. This work represents a significant step forward towards demonstrating the practical utility of DNA aptamers in clinical diagnosis.