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One-step generation of cell-laden microgels using double emulsion drops with a sacrificial ultra-thin oil shell
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论文类型: 期刊论文
第一作者: Choi, Chang-Hyung
通讯作者: Wang, HN; Weitz, DA (reprint author), Harvard Univ, John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA.
合写作者: Wang, Huanan,Lee, Hyomin,Kim, June Hwan,Zhang, Liyuan,Mao, Angelo,Mooney, David J.,Weitz, David A.
发表时间: 2016-04-26
发表刊物: LAB ON A CHIP
收录刊物: SCIE、PubMed
卷号: 16
期号: 9
页面范围: 1549-1555
ISSN号: 1473-0197
摘要: Cell-laden microgels with highly uniform sizes have significant potential in tissue engineering and cell therapy due to their capability to provide a physiologically relevant three-dimensional (3D) microenvironment for living cells. In this work, we present a simple and efficient microfluidic approach to produce monodisperse cell-laden microgels through the use of double emulsion drops with an ultra-thin oil shell as the sacrificial template. Specifically, the thin oil shell in double emulsion spontaneously dewets upon polymerization of the innermost precursor drop and subsequent transfer into an aqueous solution, resulting in direct dispersion of microgels in the aqueous phase. Compared to conventional single emulsion-based techniques for cell encapsulation, this one-step approach prevents prolonged exposure of cells to the oil phase, leading to high-throughput cell encapsulation in microgels without compromising the cell viability. Moreover, this approach allows us to culture cells within a 3D microgel which mimics the extracellular matrix, thus enabling long-term cell functionality. This microfluidic technique represents a significant step forward in high-throughput cell microencapsulation technology and offers a potentially viable option to produce cell-laden microgels for widespread applications in tissue engineering and cell therapies.

王华楠

教授   博士生导师   硕士生导师

性别: 男

毕业院校:Radboud大学奈美根医学院

学位: 博士

所在单位:生物工程学院

学科:生物化工. 生物工程与技术. 高分子材料

办公地点: 生物工程学院525

联系方式:huananwang@dlut.edu.cn

电子邮箱:huananwang@dlut.edu.cn

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