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    戴建英

    • 副教授     博士生导师   硕士生导师
    • 性别:女
    • 毕业院校:新墨西哥大学
    • 学位:博士
    • 所在单位:生物工程学院
    • 学科:生物化工. 微生物学
    • 办公地点:生物工程学院307
    • 联系方式:jydai@dlut.edu.cn
    • 电子邮箱:jydai@dlut.edu.cn

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    Separation and purification of Klebsiella phage by two-step salting-out extraction

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    论文类型:期刊论文

    第一作者:Zhang, Zhi-Rong

    通讯作者:Shen, Jun-Tao,戴建英,孙亚琴,董悦生,Xiu, Zhi-Long

    发表时间:2021-06-05

    发表刊物:SEPARATION AND PURIFICATION TECHNOLOGY

    卷号:242

    ISSN号:1383-5866

    关键字:Bacteriophage; Separation and purification; Sating-out extraction; Klebsiella pneumonia

    摘要:The bacterial resistance caused by the irrational usage of antibiotic has become a global public health crisis. Phage therapy is expected to be an alternative to antibiotics for the treatment of drug-resistant infections. However, industrial separation and purification of phage are cumbersome, time-consuming, high cost, or low efficiency. In this study, two-step salting-out extraction of Klebsiella phage was evaluated. In the first step, under the optimal conditions of 10 wt% sodium citrate/30 wt% ethyl acetate, 99 +/- 4.86% of phage was recovered in the bottom phase. Meanwhile, most of the cells and proteins could be removed. Subsequently, n-propanol was added into the bottom phase for the second salting-out extraction. Under the optimal conditions, 77 +/- 1.3% of the phage can be recovered in the intermediate phase, and the concentration factor can be as high as 475 +/- 17.6. Through the two-step salting-out extraction, the purification fold of phage to total proteins, cells and endotoxins was 19.31 +/- 1.26, 18.04 +/- 1.3 and 5.19 +/- 0.49, respectively, as well as a total phage recovery of 77 +/- 2.6%, a total concentration factor of 500 +/- 24 and no significant reduction in infectivity of phage. This work provides a potential method for industrial phage purification and has a great significance for the future development of phage therapy.