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    戴建英

    • 副教授     博士生导师   硕士生导师
    • 性别:女
    • 毕业院校:新墨西哥大学
    • 学位:博士
    • 所在单位:生物工程学院
    • 学科:生物化工. 微生物学
    • 办公地点:生物工程学院307
    • 联系方式:jydai@dlut.edu.cn
    • 电子邮箱:jydai@dlut.edu.cn

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    High acetoin production by a newly isolated marine Bacillus subtilis strain with low requirement of oxygen supply

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    论文类型:期刊论文

    第一作者:Dai, Jian-Ying

    通讯作者:Xiu, ZL (reprint author), Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China.

    合写作者:Cheng, Ling,He, Qing-Feng,Xiu, Zhi-Long

    发表时间:2015-11-01

    发表刊物:PROCESS BIOCHEMISTRY

    收录刊物:SCIE、EI、Scopus

    卷号:50

    期号:11

    页面范围:1730-1734

    ISSN号:1359-5113

    关键字:Acetoin; Marine bacteria; Optical purity; Oxygen supply

    摘要:Acetoin is a natural flavor and mainly used as a food additive and important intermediate to synthesize novel optically active compounds. In this work a new acetoin producer, Bacillus subtilis DL01 was isolated from samples of sea sediment, and its growth properties and acetoin productivity were explored. The experiments showed that the new strain can grow well in a simple fermentation medium only containing sugarcane molasses, (NH4)(2)HPO4 and MnSO4. When the initial concentration of sugar was 200 g/l in molasses, 61.15 g/l acetoin (58.2 g/l D-acetoin) was obtained with a productivity of 0.807 g/(1 h) by a batch fermentation under stage-enhanced agitation and constant aeration of 0.4 vvm. By contrast, 76 g/l acetoin (60.9 g/l D-acetoin) could be obtained with a productivity of 1.00 g/(1 h) and a yield of 0.421 g/g if 210 g/l glucose was used. The strain DL01 demonstrated high sugar tolerance, high acetoin production, and low oxygen requirement. (C) 2015 Elsevier Ltd. All rights reserved.