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    戴建英

    • 副教授     博士生导师   硕士生导师
    • 性别:女
    • 毕业院校:新墨西哥大学
    • 学位:博士
    • 所在单位:生物工程学院
    • 学科:生物化工. 微生物学
    • 办公地点:生物工程学院307
    • 联系方式:jydai@dlut.edu.cn
    • 电子邮箱:jydai@dlut.edu.cn

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    Characterization and Cofactor Binding Mechanism of a Novel NAD(P)H-Dependent Aldehyde Reductase from Klebsiella pneumoniae DSM2026

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    论文类型:期刊论文

    第一作者:Ma, Cheng-Wei

    通讯作者:Dai, JY (reprint author), Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China.

    合写作者:Zhang, Le,Dai, Jian-Ying,Xiu, Zhi-Long

    发表时间:2013-12-01

    发表刊物:JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

    收录刊物:SCIE、PubMed、Scopus

    卷号:23

    期号:12

    页面范围:1699-1707

    ISSN号:1017-7825

    关键字:1,3-Propanediol; aldehyde reductase; cofactor binding; free-energy decomposition; Klebsiella pneumoniae

    摘要:During the fermentative production of 1,3-propanediol under high substrate concentrations, accumulation of intracellular 3-hydroxypropionaldehyde will cause premature cessation of cell growth and glycerol consumption. Discovery of oxidoreductases that can convert 3-hydroxypropionaldehyde to 1,3-propanediol using NADPH as cofactor could serve as a solution to this problem. In this paper, the yqhD gene from Klebsiella pneumoniae DSM2026, which was found encoding an aldehyde reductase (KpAR), was cloned and characterized. KpAR showed broad substrate specificity under physiological direction, whereas no catalytic activity was detected in the oxidation direction, and both NADPH and NADH can be utilized as cofactors. The cofactor binding mechanism was then investigated employing homology modeling and molecular dynamics simulations. Hydrogen-bond analysis showed that the hydrogen-bond interactions between KpAR and NADPH are much stronger than that for NADH. Free-energy decomposition dedicated that residues Gly37 to Val41 contribute most to the cofactor preference through polar interactions. In conclusion, this work provides a novel aldehyde reductase that has potential applications in the development of novel genetically engineered strains in the 1,3-propanediol industry, and gives a better understanding of the mechanisms involved in cofactor binding.