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论文类型：期刊论文

发表时间：2010-11-01

发表刊物：BIORESOURCE TECHNOLOGY

收录刊物：PubMed、SCIE、EI、Scopus

卷号：101

期号：21

页面范围：8342-8347

ISSN号：0960-8524

关键字：2,3-Butanediol; Lignocellulose; Jerusalem artichoke; Simultaneous saccharification and fermentation; Stage-shift aeration

摘要：Jerusalem artichoke stalk and tuber can serve as a low cost feedstock for the production of 2,3-butanediol. However, like other lignocellulosic materials, the sugar concentration in the hydrolysate prepared from Jerusalem artichoke stalk is too low to be utilized effectively by microorganisms. In this paper a novel strategy was proposed to increase the sugar concentration by adding Jerusalem artichoke tuber into the hydrolysate of the stalk. The sugar was then biotransformed into high-valued 2,3-butanediol by Klebsiella pneumoniae. Fed-batch simultaneous saccharification and fermentation (SSF) was effectively performed and 901.2 mmol/l (80.5 g/l) target products (2,3-butanediol plus acetoin) was obtained in 68 h by a stage-shift aeration strategy. The concentration, yield and productivity of target products were 16.9%, 16.8% and 23.4%, respectively, higher than the best results obtained with SSF operated under constant aeration. This showed that adding tuber to the stalk hydrolysate was a useful strategy for increasing the production of 2,3-butanediol from Jerusalem artichoke via fermentation. (C) 2010 Elsevier Ltd. All rights reserved.