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副教授

硕士生导师

性别:男

毕业院校:大连理工大学

学位:博士

所在单位:化工学院

学科:化学工程. 生物医学工程. 生物化工

办公地点:大连理工大学化工学院化工实验楼D413

联系方式:139玖捌伍肆捌柒壹柒

电子邮箱:guanshui@dlut.edu.cn

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Chitosan/gelatin porous scaffolds assembled with conductive poly(3,4-ethylenedioxythiophene) nanoparticles for neural tissue engineering

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论文类型:期刊论文

第一作者:Wang, Shuping

通讯作者:Guan, S (reprint author), Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China.

合写作者:Sun, Changkai,Guan, Shui,Li, Wenfang,Xu, Jianqiang,Ge, Dan,Zhuang, Meiling,Liu, Tianqing,Ma, Xuehu

发表时间:2017-06-28

发表刊物:JOURNAL OF MATERIALS CHEMISTRY B

收录刊物:SCIE、EI

卷号:5

期号:24

页面范围:4774-4788

ISSN号:2050-750X

摘要:Electroactive biomaterials are widely explored as scaffolds for nerve tissue regeneration. Poly(3,4ethylenedioxythiophene) (PEDOT) is a conductive polymer that has been chosen to construct tissue engineered scaffolds because of its excellent conductivity and non-cytotoxicity. In the present study, an electrically conductive scaffold was prepared by assembling PEDOT on a chitosan/gelatin (Cs/Gel) porous scaffold surface via in situ interfacial polymerization. The hydrophilic Cs/Gel hydrogel was used as a template, and PEDOT nanoparticles were uniformly assembled on the scaffold surface. The static polymerization of the 3,4-ethylenedioxythiophene (EDOT) monomer at the interface between the aqueous phase and the organic phase was accompanied by the formation of the PEDOT-assembled Cs/Gel scaffolds. PEDOT/Cs/Gel scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. The results confirmed the deposition of PEDOT nanoparticles with the mean diameter of 50 nm on the Cs/Gel scaffold channel surface. Compared to the Cs/Gel scaffold, the incorporation of PEDOT on the scaffold increased the electrical conductivity, hydrophilicity, mechanical properties and thermal stability, whereas decreased the water absorption and biodegradation. For biocompatibility, PEDOT/Cs/Gel scaffolds, especially the 2PEDOT/Cs/Gel scaffold group, significantly promoted neuron-like rat pheochromocytoma (PC12) cell adhesion and proliferation. The results of both the gene expression and protein level assessments suggested that the PEDOT-assembled Cs/Gel scaffold enhanced the PC12 cellular neurite growth with higher protein and gene expression levels. This is the first report on the construction of a conductive PEDOT/Cs/Gel porous scaffold via an in situ interfacial polymerization method, and the results demonstrate that it may be a promising conductive scaffold for neural tissue engineering.