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论文类型：期刊论文

第一作者：Wang, Shuping

通讯作者：Guan, S (reprint author), Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian, Peoples R China.

合写作者：Guan, Shui,Li, Wenfang,Ge, Dan,Xu, Jianqiang,Sun, Changkai,Liu, Tianqing,Ma, Xuehu

发表时间：2018-12-01

发表刊物：MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

收录刊物：PubMed、SCIE、Scopus

卷号：93

页面范围：890-901

ISSN号：0928-4931

关键字：Conducting polymer; 3D culture; Neural stem cell; Proliferation; Differentiation

摘要：Neural stem cells (NSCs), as a self-renewing and multipotent cell population, have been widely studied for never regeneration. Engineering scaffold is one of the important factors to regulate NSCs proliferation and differentiation towards the formation of the desired cells and tissues. Because neural cells are electro-active ones, a conductive scaffold is required to provide three-dimensional cell growth microenvironments and appropriate synergistic cell guidance cues. In this study, a poly (3,4-ethylenedioxythiophene)/chitosan/gelatin (PEDOT/Cs/Gel) scaffold was prepared via in situ interfacial polymerization, with a nanostructured layer of PEDOT assembling on the channel surface of porous Cs/Gel scaffold. This electrically conductive, three-dimensional, porous and biodegradable PEDOT/Cs/Gel scaffold was used as a novel scaffold for NSCs three-dimension (3D) culture in vitro. It was found that the layer of PEDOT on the channel surface of Cs/Gel scaffolds could greatly promote NSCs adhesion and proliferation. Additionally, under the differentiation condition, the protein and gene analysis suggested that PEDOT/Cs/Gel scaffolds could significantly enhance the NSCs differentiation towards neurons and astrocytes with the up-regulation of beta tubulin-III and GFAP expression. In conclusion, these results demonstrated that the PEDOT/Cs/Gel scaffolds as an electrically conductive scaffold could not only promote NSCs adhesion and proliferation but also enhance NSCs differentiation into neurons and astrocytes with higher protein and gene expression. PEDOT-assembled Cs/Gel scaffold will be a promising conductive substrate for NSCs research and neural tissue engineering.