郭兆明

个人信息Personal Information

副教授

硕士生导师

性别:女

毕业院校:北京大学

学位:博士

所在单位:化工海洋与生命学院

学科:药剂学

电子邮箱:guozm@dlut.edu.cn

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Dual targeting mesoporous silica nanoparticles for inhibiting tumour cell invasion and metastasis

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论文类型:期刊论文

发表时间:2017-12-20

发表刊物:INTERNATIONAL JOURNAL OF PHARMACEUTICS

收录刊物:Scopus、SCIE、PubMed

卷号:534

期号:1-2

页面范围:71-80

ISSN号:0378-5173

关键字:ADH-1; Hyaluronic acid; Mesoporous silica nanoparticles; Drug delivery; Invasion and metastasis

摘要:The invasion and metastasis of tumour cells are closely correlated with poor prognosis of cancer patients. In this study, a CD44 and N-cadherin dual targeting drug delivery system based on mesoporous silica nanoparticles (MSNs) has been successfully constructed for inhibiting tumour cell invasion and metastasis. Amino modified MSN (MSN/NH2) was first synthesized and then functionalized with hyaluronic acid (HA) and ADH-1, constructing the carrier ADH-1-HA-MSN. Doxorubicin hydrochloride (DOX) was selected as a model anticancer drug. The prepared vector had a spherical shape with a narrow distribution of particle size. Flow cytometry and confocal microscopy studies showed that the modification with HA significantly enhanced CD44-mediated cellular uptake of this nanocarrier. ADH-1-HA-MSN/DOX exhibited higher cytotoxicity compared to non-ADH-1 modified counterparts. Of note, a transwell chamber assay demonstrated that the migration and invasion of tumour cells were markedly inhibited by ADH-1-HA-MSN/DOX. Furthermore, Western blotting analysis revealed that ADH-1-HA-MSN/DOX inhibited tumour cell invasion and metastasis by down-regulating N-cadherin expression. Taken together, these results indicated that ADH-1-HA-MSN might be a promising targeted drug delivery system for inhibiting cancer invasion and metastasis.