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论文类型：期刊论文

发表时间：2018-06-01

发表刊物：MICROCHIMICA ACTA

收录刊物：PubMed、SCIE

卷号：185

期号：6

页面范围：291

ISSN号：0026-3672

关键字：Manganese dioxide/carbon based dots nanohybrids; Oxidation; Tetramethylbenzidine; Fatel calf serum sample; Visual detection

摘要：A kind of single-layer carbon based dots (CDs) with abundant carboxyl functional groups was hybridized with manganese dioxide (MnO2). The resulting nanohybrid is stable and can be well dispersed in water. MnO2 is capable of oxidizing the substrate 3,3'5,5'-tetramethylbenzidine (TMB) to form a blue product whose absorption (peaking at 655 nm) fades in the presence of glutathione (GSH). A sensitive and selective colorimetric GSH assay was worked out that has a linear response in the 10 to 0.1 mu M GSH concentration range, with a 0.095 mu M detection limit. The method was applied to the determination of GSH in spiked fetal calf serum where it gave excellent recoveries.