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论文类型：期刊论文

发表时间：2017-12-01

发表刊物：ENVIRONMENTAL POLLUTION

收录刊物：SCIE、EI、PubMed

卷号：231

期号：Pt 1

页面范围：1145-1152

ISSN号：0269-7491

关键字：Antibiotic resistance genes; Polycyclic aromatic hydrocarbons; Integrons; Horizontal genetic transfer; Conjugative transfer

摘要：Antibiotic resistance genes (ARGs) have been regarded as emerging contaminants and have attracted growing attention owing to their widespread presence in the environment. In addition to the well documented selective pressure of antibiotics, ARGs have also become prevalent because of anthropogenic impacts. Coastal habitats are located between terrestrial and marine ecosystems, which are a hotspot for anthropogenic impacts. Excessive accumulation of polycyclic aromatic hydrocarbons (PAHs) has posed a serious threat to coastal habitats, but no information is available on the effect of PAHs on antibiotic resistance in the microbial community of coastal environments. In this study, the effect of two typical PAHs, naphthalene and phenanthrene, on antibiotic resistance propagation was investigated in a coastal microbial community. The results indicated that the presence of 100 mg/L of naphthalene or 10 mg/L of phenanthrene significantly enhanced the abundance of class I integrase gene (Win sulfanilamide resistance gene (sull), and aminoglycosides resistance gene (aadA2) in the microbial community. Horizontal gene transfer experiment demonstrated that increased abundance of ARGs was primarily a result of conjugative transfer mediated by class I integrons. These findings provided direct evidence that coastal microbial community exposed to PAHs might have resulted in the dissemination of ARGs and implied that a more comprehensive risk assessment of PAHs to natural ecosystems and public health is necessary. (C) 2017 Elsevier Ltd. All rights reserved.