柳广飞

个人信息Personal Information

副教授

博士生导师

硕士生导师

性别:男

毕业院校:大连理工大学

学位:博士

所在单位:环境学院

学科:环境工程. 环境科学

办公地点:环境学院711室

联系方式:0411-84706252

电子邮箱:guangfeiliu@dlut.edu.cn

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Decolorization of azo dyes by Geobacter metallireducens

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论文类型:期刊论文

发表时间:2013-09-01

发表刊物:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录刊物:SCIE、EI、PubMed、Scopus

卷号:97

期号:17

页面范围:7935-7942

ISSN号:0175-7598

关键字:Azo dye; Decolorization; Humic acid; Lawsone; Geobacter metallireducens

摘要:Geobacter metallireducens was found to be capable of decolorizing several azo dyes with different structures to various extents. Pyruvate, ethanol, acetate, propionate, and benzoate could support 66.3 +/- 2.6-93.7 +/- 2.1 % decolorization of 0.1 mM acid red 27 (AR27) in 40 h. The dependence of the specific decolorization rate on AR27 concentration (25 to 800 mu M) followed Michaelis-Menten kinetics (K (m) = 186.9 +/- 1.4 mu Ie, V (max) = 0.65 +/- 0.02 mu mol mg protein(-1) h(-1)). Enhanced AR27 decolorization was observed with the increase of cell concentrations ranging from 7.5 to 45 mgL(-1). AR27 decolorization by G. metallireducens was retarded by the presence of goethite, which competed electrons with AR27 and was reduced to Fe(II). The addition of low concentrations of humic acid (1-100 mgL(-1)) or 2-hydroxy-1,4-naphthoquinone (0.5-50 mu M) could improve the decolorization performance of G. metallireducens. High-performance liquid chromatography analysis suggested reductive pathway to be responsible for decolorization. This was the first study on azo dye decolorization by Geobacter strain and might improve our understanding of natural attenuation and bioremediation of environments polluted by azo dyes.