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论文类型：期刊论文

发表时间：2013-03-01

发表刊物：COLLOIDS AND SURFACES B-BIOINTERFACES

收录刊物：SCIE、EI、PubMed

卷号：103

页面范围：38-44

ISSN号：0927-7765

关键字：Immunosensor; Microcystin-LR; Graphene; Carbon nanosphere; Toxins

摘要：A graphene-based immunosensor was fabricated for the detection of microcystin-LR using a horseradish peroxidase-carbon nanosphere-antibody system for signal amplification. Graphene and chitosan were used as immobilization materials to improve the electrochemical performance of the electrode. Signal amplification was achieved using multienzyme functionalized carbon nanosphere. This approach provided a linear range from 0.05 to 15 mu g L-1 microcystin-LR with a detection limit of 0.016 mu g L-1. Analytical detection of microcystin-LR in environmental water samples were demonstrated by comparing the results obtained using immunosensor and high-performance liquid chromatography. This electrochemical immunosensor showed good performance with high sensitivity, acceptable stability, repeatability and the potential for use in routine water quality monitoring for various toxins. (c) 2012 Elsevier B.V. All rights reserved.