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发表时间：2020-01-01

发表刊物：Journal of Dalian University of Technology

所属单位：环境学院

卷号：60

期号：6

页面范围：562-569

ISSN号：1000-8608

关键字："SNAD-IFAS; landfill leachate; anaerobic digestion; SNAD-IFAS; carbon and nitrogen removal; microbial community structure"

CN号：21-1117/N

摘要：An integrated configuration combining up-flow anaerobic sludge bed(UASB)with the simultaneous partial nitrification,anammox and denitrification-integrated fixed biofilm activated sludge(SNAD-IFAS)was constructed for the treatment of landfill leachate.The temperature and pH of UASB were controlled at 32℃and 8.0-8.3,respectively.The removal efficiency of COD reaches 44.9% when the hydraulic retention time(HRT)is 24hand the C and N ratio is around 1.3∶1 which could meet the requirement of continued SNAD process completely.The two-stage tandem-type SNAD-IFAS reactors were operated for 96dand it used the diluted effluent of UASB as the influent. The temperature,DO concentration and pH were regulated independently as 32℃,$0.1 - 0.2\;{\rm{mg}} \cdot {{\rm{L}}^{ - 1}}$ and 7.8-8.0in SNAD1pond,as 32℃,$0.08 - 0.14\;{\rm{mg}} \cdot {{\rm{L}}^{ - 1}}$ and 7.5-7.8in SNAD2pond.In stable phase,the TN and COD removal efficiencies of SNAD process are 83.3% and 39.4% with the concentration of TN and COD reaches $602.3\;{\rm{mg}} \cdot {{\rm{L}}^{ - 1}}$and $878.1\;{\rm{mg}} \cdot {{\rm{L}}^{ - 1}}$,respectively.The result of high-throughput pyrosequencing indicates that the typical microbial community structure of SNAD process is formed in the reactors.Ammonium oxidizing bacteria(AOB)tends to grow in the suspended sludge while the anaerobic ammonium oxidizing bacteria(AnAOB)enriches in biofilms. The biofilms in SNAD1pond and SNAD2pond are both dominated by AnAOB,whose abundance attains 26.21%and 30.82%,respectively.

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