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论文类型： 期刊论文

发表时间： 2008-05-01

发表刊物： BIORESOURCE TECHNOLOGY

收录刊物： SCIE、PubMed

卷号： 99

期号： 8

页面范围： 2749-2756

ISSN号： 0960-8524

关键字： membrane-aerated biofilm reactor; microbial community; anammox; nitrification; biofilm

摘要： The 16S rDNA-based molecular technique was applied to investigate the functional microbial community of a membrane-aerated biofilm (MAB) that was used for completely autotrophic nitrogen removal over nitrite (CANON). The relationships among two kinds of key bacteria responsible for CANON: aerobic ammonia-oxidizing bacteria (AOB) and Anammox bacteria, and their possible distributions in the MAB were discussed based on the microbial community analysis. FISH analysis showed the existence of two visible active layers in experimental MAB. One is the partial nitrifying layer located in the region of oxygen-rich membrane-biofilm interface, dominated by NSO190-positive AOB. The other is the Anammox active layer located in the region of anoxic liquid-biofilm interface, dominated by PLA46 and AMX820-positive Anammox microorganisms. As a result of this study, the AOB as well as Anammox bacteria were present and active in experimental MABR, and the cooperation between AOB and Anammox bacteria was considered to be responsible for CANON. (c) 2007 Elsevier Ltd. All rights reserved.