Title of Paper:Isolation and characterization of an Alcaligenes sp strain DG-5 capable of degrading DDTs under aerobic conditions

Hits:

Date of Publication:2011-07-01

Journal:JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES

Included Journals:SCIE、PubMed、Scopus

Volume:46

Issue:3

Page Number:257-263

ISSN No.:0360-1234

Key Words:DDTs; biodegradation; aerobic degradation; Alcaligenes sp.

Abstract:In this study, an Alcaligenes sp. strain DG-5 that can effectively degrade dichlorodiphenyltrichloro-ethanes (DDTs) under aerobic conditions was isolated from DDTs-contaminated sediment. Various factors that affect the biodegradation of DDTs by DG-5 were investigated. About 88 %, 65 % and 45 % of the total DDTs were consumed within 120 h when their initial concentrations were 0.5, 5 and 15 mg L(-1), respectively. However, almost no degradation was observed when their concentration was increased to 30 mg L(-1), but the addition of nutrients significantly improved the degradation, and 66 % and 90 % of the total DDTs were degraded at 336 h in the presence of 5 g L(-1) peptone and yeast extract, respectively. Moreover, the addition of 20 mM formate also enhanced the ability of DG-5 to transform DDTs, and its DDT transformation capacity (T(c)) value was increased by 1.8 - 2.7 fold for the pure (p,p'-DDT or o,p'-DDT only) and mixed systems (p,p'-DDT, o,p'-DDT, p,p'-DDD and p,p'-DDE). Furthermore, it was found that competitive inhibition in the biodegradation by DDT compounds occurred in the mixed system.