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Indexed by:Journal Papers

Date of Publication:2015-05-01

Journal:REVIEW OF SCIENTIFIC INSTRUMENTS

Included Journals:SCIE、EI、PubMed、Scopus

Volume:86

Issue:5

Page Number:053105

ISSN No.:0034-6748

Abstract:Knowledge of dynamical structure of protein is an important clue to understand its biological function in vivo. Temperature-jump (T-jump) time-resolved transient mid-IR absorbance spectroscopy is a powerful tool in elucidating the protein dynamical structures and the folding/unfolding kinetics of proteins in solution. A home-built setup of T-jump time-resolved transient mid-IR absorbance spectroscopy with high sensitivity is developed, which is composed of a Q-switched Cr, Tm, Ho:YAG laser with an output wavelength at 2.09 mu m as the T-jump heating source, and a continuous working CO laser tunable from 1580 to 1980 cm(-1) as the IR probe. The results demonstrate that this system has a sensitivity of 1 x 10(-4) Delta OD for a single wavelength detection, and 2 x 10(-4) Delta OD for spectral detection in amide I' region, as well as a temporal resolution of 20 ns. Moreover, the data quality coming from the CO laser is comparable to the one using the commercial quantum cascade laser. (C) 2015 AIP Publishing LLC.