摘要：A surface plasmon resonance (SPR) biochemical analyzer is introduced.The method of combining angle scanning with intensity modulation is adopted to realize the real-time monitoring of glycoprotein.In the experiments of glycoprotein detection, the renewable solution of pH=3 is chosen because the regenerative capacity of the solution reaches 97% in the renewable solution of different pH comparison.Relative standard deviation (RSD) is 3.83% in the five measurements of the same concentration glycoprotein.The experimental method can detect the specificity of glycoprotein in the comparative experiments of non-glycoprotein and glycoprotein.Finally, the glycoprotein concentration between 0.01 mg/mL and 1 mg/mL is detected.The relationship between the concentration of RNase B and the normalized light intensity is more consistent with polynomial fitting by comparing the residual analysis and correlation coefficients of linear fitting and polynomial fitting, and polynomial fitting curve is used as standard curve.The experimental results show that the SPR spectrometer can detect the specificity of glycoprotein, while will be widely used in medical field.
