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透明颤菌血红蛋白基因克隆及在紫色非硫细菌中的表达

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Indexed by:期刊论文

Date of Publication:2008-01-15

Journal:河北工业科技

Volume:25

Issue:1

Page Number:1-4

ISSN No.:1008-1534

Key Words:透明颤菌血红蛋白基因;天然启动子;光合细菌;基因工程菌;低氧诱导

Abstract:构建了含有透明颤菌血红蛋白基因的可转化大肠杆菌-紫色非硫细菌穿梭质粒(pHSG-MG-vgb),将其转化到紫色非硫细菌(Rhodopseudo palustns),获得了基因工程菌EPB01.经SDS-PAGE和CO示差分光光谱分析证明,重组菌株表达了有活性的透明颤菌血红蛋白,通过低溶解氧诱导后,基因工程菌EPB01的菌体密度较对照菌增加了24%~32%.

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