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Indexed by:期刊论文

Date of Publication:2012-01-01

Journal:BIOTECHNOLOGY LETTERS

Included Journals:SCIE、EI、PubMed

Volume:34

Issue:1

Page Number:117-123

ISSN No.:0141-5492

Key Words:2,3-Dihydroxybiphenyl-1,2-dioxygenase; Homology modeling; Metagenome; Molecular docking

Abstract:A 2,3-dihydroxybiphenyl-1,2-dioxygenase gene (designated as bphC_meta) was identified in activated sludge metagenome by PCR. This gene shared 99% sequence identity with BphC from Burkholderia xenovorans LB400. The enzyme was purified from recombinant Escherichia coli with a subunit molecular mass of 32 +/- A 1 kDa. It was optimally active at pH 9.0 and 40A degrees C, using 2,3-dihydroxybiphenyl as a substrate. Activity toward substituted catechols was: 2,3-dihydroxybiphenyl > 3-methylcatechol > catechol > 4-chlorocatechol (4-methylcatechol). The prediction made by molecular docking was consistent with the kinetic experimental data, and further explained the substrate preference of BphC_meta. The present study could pave the way for the improved understanding and application of BphCs derived from metagenomes.