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Indexed by:期刊论文

Date of Publication:2009-12-01

Journal:12th Asia-Pacific-Confederation-of-Chemical-Engineering (APCChE 2008)

Included Journals:SCIE、CPCI-S、PubMed

Volume:159

Issue:3

Page Number:728-738

ISSN No.:0273-2289

Key Words:Azo dye; Decolorization; Salt-tolerant; Redox mediator

Abstract:A salt-tolerant bacterium was isolated from the surface soil of a pharmaceutical factory, which could efficiently decolorize azo dyes. The strain was identified as Exiguobacterium sp. according to its morphological characteristics and 16S rRNA gene sequence analysis. Decolorization of X-3B with resting cells of this strain, which were catalyzed by redox mediator (anthraquinone), was studied, and the conditions were optimized. For color removal and cells growth, the optimal inoculation amount, pH, temperature, salinity, and metal ions were 6% (v/v), 5.4-7.0, 30-40 A degrees C, 15% (w/v) NaCl, and 1 mmol L(-1) Mg(2+) or Ca(2+), respectively. It was exhibited that decolorization process proceeded primarily by enzymatic reduction associated with a minor portion of bio-adsorption to inactivated microbial cells. Anthraquinone could really accelerate the decolorization of X-3B under the optimal conditions.