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Indexed by:期刊论文

Date of Publication:2013-06-01

Journal:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Included Journals:SCIE、EI、Scopus

Volume:97

Issue:11

Page Number:5097-5103

ISSN No.:0175-7598

Key Words:Biotransformation; Phenol hydroxylase; Catechol; Toluene; Benzene

Abstract:Phenol hydroxylase gene engineered microorganism (PHIND) was used to synthesize catechols from benzene and toluene by successive hydroxylation reaction. HPLC-MS and H-1 NMR analysis proved that the products of biotransformation were the corresponding catechols via the intermediate production of phenols. It was indicated that the main products of toluene oxidation were o-cresol and p-cresol. 3-Methylcatechol was the predominant product for m-cresol biotransformation. Formation rate of catechol (25 mu M/min/g cell dry weight) was 1.43-fold higher than that of methylcatechols. It was suggested that phenol hydroxylase could be successfully used to transform both benzene and toluene to catechols by successive hydroxylation.