Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2013-06-01

Journal: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Included Journals: Scopus、EI、SCIE

Volume: 97

Issue: 11

Page Number: 5097-5103

ISSN: 0175-7598

Key Words: Biotransformation; Phenol hydroxylase; Catechol; Toluene; Benzene

Abstract: Phenol hydroxylase gene engineered microorganism (PHIND) was used to synthesize catechols from benzene and toluene by successive hydroxylation reaction. HPLC-MS and H-1 NMR analysis proved that the products of biotransformation were the corresponding catechols via the intermediate production of phenols. It was indicated that the main products of toluene oxidation were o-cresol and p-cresol. 3-Methylcatechol was the predominant product for m-cresol biotransformation. Formation rate of catechol (25 mu M/min/g cell dry weight) was 1.43-fold higher than that of methylcatechols. It was suggested that phenol hydroxylase could be successfully used to transform both benzene and toluene to catechols by successive hydroxylation.