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Metabolic characterization and genes for the conversion of biphenyl in Dyella ginsengisoli LA-4

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Indexed by:期刊论文

Date of Publication:2012-02-01

Journal:BIOTECHNOLOGY AND BIOENGINEERING

Included Journals:SCIE、EI、PubMed、Scopus

Volume:109

Issue:2

Page Number:609-613

ISSN No.:0006-3592

Key Words:Dyella ginsengisoli; biphenyl; bph gene cluster

Abstract:A complete bph gene cluster (bphLA-4) containing 12,186?bp was amplified from Dyella ginsengisoli LA-4. The bphLA-4 was composed of bphABCXD, and an additional gene encoding a meta-fission product hydrolase was located in the bphX region. BphLA-4 was independently transcribed by the two operons, bphA1A2orf1A3A4BCX0 and bphX1orf2X2X3D, and significantly differed from bphKF707. Both benzoate and catechol induced the expression of both operons. 2-Hydroxypenta-2,4-dienoate was identified as the intermediate of the biphenyl degradation by strain LA-4. This finding suggested that there existed a novel lower pathway of biphenyl degradation in strain LA-4. Biotechnol. Bioeng. 2012; 109:609613. (c) 2011 Wiley Periodicals, Inc.

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