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Indexed by:期刊论文

Date of Publication:2012-12-01

Journal:ENVIRONMENTAL TECHNOLOGY

Included Journals:SCIE、PubMed、Scopus

Volume:33

Issue:23

Page Number:2603-2609

ISSN No.:0959-3330

Key Words:Streptomyces; alkali lignin; biodegradation; co-culture; GC-MS analysis

Abstract:Biodegradation and bioconversion of lignin are the result of the combined action of fungi, bacteria and actinomycetes. Through screening from forest soil, two novel isolated actinomycete strains were identified as Streptomyces spp. strains F-6 and F-7 by their morphology, cultural characteristics and high homology to the 16S rRNA gene. Both strains possessed laccase and manganese peroxidase activities. Laccase activity produced by strain F-6 was up to 935.4Ug(-1) dry cell weight. More than 50% of alkali lignin was removed by strains F-6 and F-7 in 12 days of incubation. GC-MS analysis of the biodegraded products showed strain F-6 converted lignin into phenol and broken phenol compounds. The two strains could co-culture with white-rot fungus, and the combined actinonycete-fungus system decomposed alkali lignin effectively.